Foxj1-EGFP-CreERT2

系統名

C57BL/6Smoc-Foxj1em1(GFP-CreERT2-polyA)Smoc

SMOC番号

NM-KI-200133

維持形態

Repository Live

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遺伝子の概要

Gene Symbol
Foxj1

Synonyms

Hfh4; HFH-4; FKHL-13

NCBI ID

15223

MGI ID

1347474

Ensembl ID

ENSMUSG00000034227

Pubmed

Foxj1

Human Ortholog

FOXJ1

説明

A GFP-CreERT2-polyA expression cassette was knocked into the Foxj1 gene start codon site.
応用分野：The expression pattern of GFP-CreERT2 was similar to the endogenous pattern of Foxj1 gene expression. Tamoxifen-induced Cre recombinase activity was detected in the lung and brain. The mouse model can be used for studying hydrocephalus.

表現型データ


Foxj1-GFP-CreERT2-1.png

Fig.1 CreERT2-mediated recombination in the lung of Foxj1CreERT2/+; Rosa26tdTomato/+ mouse. 

TdTomato+ cells (red) were colocalized with GFP+ cells (green) in the bronchial epithelial cells of Foxj1CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. Some leakiness were detected in the lung prior to tamoxifen exposure.

Foxj1-GFP-CreERT2-2.png

Fig.2 CreERT2-mediated recombination in the brain of Foxj1CreERT2/+; Rosa26tdTomato/+ mouse. 

TdTomato(red) expression can be detected in the choroid plexus of Foxj1CreERT2/+; Rosa26tdTomato/+ mouse after tamoxifen treatment. Some leakiness were detected in the choroid plexus prior to tamoxifen exposure. EGFP(green) expression can not be observed.

Foxj1-GFP-CreERT2-3.png

Fig.3 Detection of tdTomato (red) and GFP（green）in various tissues of Foxj1CreERT2/+; Rosa26tdTomato/+ mice. CreERT2 mediated recombination can not be detected in the thymus, heart, lung, stomach, submandibular gland, brown fats, kidney, pancreas, intestine, colon, spleen, ovary, uterus, muscle or skin of female mice(12-13 weeks old). (For more detailed information please contact our technical advisor.)

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